Fluorochrome spectra
WebApr 14, 2024 · The Excitation and Emission spectra. Fluorochromes absorb and emit light at varying intensities across the spectrum, with each fluorochrome having distinct absorption and emission spectra. These spectra indicate that a specific fluorochrome can absorb adjacent wavelengths, but its maximum absorption occurs in the peak … WebUse the new interactive SpectraViewer tool to select the optimal fluorescent dyes for your experiment. Easily compare excitation and emission spectra for multiple products for …
Fluorochrome spectra
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WebPE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. WebBrightness of various fluorochrome conjugates Freshly isolated lymphocytes, stained with anti-human CD4 (RPA-T4) conjugated with various fluorochromes run on a BD LSR II flow cytometer. The fluorochromes were ranked based on observed stain index values. This chart is meant as a guideline of relative stain indices of various fluorochromes.
WebFluorochrome Guides Cytek’s fluorochrome guides provide examples of many dyes full spectrum signatures along with stain indices, spread information, and other useful … WebThis is a table of some characteristics of fluorochromes useful for flow cytometry or fluorescence microscopy. Within groups, roughly in order of excitation wavelength (families excepted). Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself.
WebA fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain … WebTo help with pinpointing exact data, the analyzer has the capacity to zoom in, allowing you easily visualize spectra intersection points with laser lines, filter sets and other spectra. …
WebSpectra viewers and fluorochrome reference charts are useful tools to determine the maximum excitation and emission wavelengths of the fluorochromes under …
WebFluorochromes have spectral characteristics that allow them to absorb light of certain wavelengths and then to emit light at longer wavelengths. Emission, a specific characteristic of the compound, is not limited to a fixed wavelength but rather constitutes a spectrum, with variable numbers of photons emitted at different wavelengths. harvest right scientific freeze dryer manualWebConcentration: 0.2 mg/ml RRID: AB_2871436 Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage Store undiluted at 4°C and protected from … harvest right set up instructionsWeb164 rows · This is a table of some characteristics of fluorochromes useful for flow … harvest right scientific freeze dryerWebDriven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). … harvest right set up testWebFor fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Cy is a trademark of Amersham Biosciences Limited. BD APC-H7 is a tandem conjugate and an analog of APC-Cy7 with the same spectral properties. books by robert borkWebOur fluorochrome chart includes Aligned emission and excitation fluorescence spectra for 30 of the most commonly used fluorochromes, including tandem dyes Easy visualization of some of the most popular … books by robert henderson at amazonWebFluorescence is the process of light emission by a substance (fluorochrome) that has previously absorbed electromagnetic radiation. Each fluorochrome has a unique and … books by robert goldsborough