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Topflash实验方法

WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … Webtopflash tcf lef luciferase reporter construct - by Bioz Stars , 2024-04 86 / 100 stars Images 1) Product Images from "Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation" Article Title: Small molecule inhibitors of WNT/β-catenin signaling block IL-1β- and TNFα-induced cartilage degradation

Technology - Super-Top-Flash Luciferase Reporter Cell Line for ...

WebTOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash containing mutated TCF binding sites is also available as a ... build cadillac ct5 v blackwing https://mycountability.com

TOPFLASH/FOPFLASH_百度知道

WebTOPFlash reporter luciferase activities were measured. The relative luciferase activity (fold-change) was reported. * P 0.05 versus controls ( n = 5, t test). ( O – R ) Overexpression of IGF2BP3 triggers the upregulation of KIM-1, p-H3, and p16 INK4A expression in tubular cells. HKC-8 cells were infected with either control lentivirus or ... WebPlasmid M51 Super 8x FOPFlash (TOPFlash mutant) from Dr. Randall Moon's lab contains the insert mutant TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is available through Addgene. Web一般而言,topflash/fopflash是比较常用的检测β-catenin的系统,原理和所有的RLU一样,都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。如果 … build cadillac suv

Supplementary Information Materials and Methods - Journal of …

Category:TOP/FOP-FLASH assay in HEK293T cells? ResearchGate

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Topflash实验方法

TCF Reporter Plasmid Kit 17-285

Web25. máj 2024 · TOPFlash载体 基本信息 订购信息 质粒图谱 载体描述 The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate … Web用western和免疫荧光验证beta-catenin和下游靶基因的变化只是基础,只是说明你转染了这个抑癌基因的确是改变了catenin通路,但是你想要证明该抑癌基因是通过catenin通路来 …

Topflash实验方法

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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96420 WebPlasmid M50 Super 8x TOPFlash from Dr. Randall Moon's lab contains the insert TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view ...

Web25. máj 2024 · 载体描述. Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPFLASH (Catalog # 21-170) which contains wild type Tcf binding sites. WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is …

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WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … buildcalWeb1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板,在其多克隆位点插入两组TCF/LEF结合位点序列,每组有三个重复序列,一组为正向序列,另一组是它的反向 … crossword christmas dinner foodWebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … crossword christmas entreesWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... crossword christian church.orghttp://www.bioon.com.cn/doc/showarticle.asp?newsid=96515 crossword christian church tvWebI did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented … crossword christian church givinghttp://www.bioon.com.cn/doc/showarticle.asp?newsid=96515 crossword christmas carol